Uji Infektivitas Tobacco Mosaic Virus (TMV) dan Cucumber Mosaic Virus (CMV) pada Chaenopodium amaranticolor
Abstract
The infectivity test is one of the completeness in knowing the physical properties of the virus. Determination of the physical properties of the virus can be done by: Thermal inactivation point (TIP), in vitro resistance (Longevity in vitro / LIV), and dilution end point (DEP). The purpose of this study was to determine the infectivity of TMV and CMV in sap through LIV, DEP, and TIP.LIV testing is done by sap incubation until the test time (0 hours, 6 hours, and 24 hours), DEP test is done by diluting sap (not diluted, 10-1, 10-2, 10-3, 10-4, 10-5, 10-6, 10-7), while TIP test is done by heating sap at room temperature, 50oC, 70oC, 90oC for 10 minutes. The results of this study show TMV is stable compared to CMV.TMV is still infectious in sap storage for 24 hours, while CMV is not infectious between 6-24 hours after incubation. The cutoff point of sap TMV dilution is between 10-5 and 10-6, while CMV has been unable to cause symptoms in indicator plants at dilutions between 10-1 and 10-2. TMV is still infectious at 90oC temperature treatment, while CMV inactivation hot spots are 40oC and 50oC.
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Agrios, G.N. 2005. Plant Pathology. Edisi ke-4. Academic Press. New York.
El-Baz, Reham M. 2004. Physicochemical, physiological and histopathological studies on cucumber mosaic virus. [Thesis]. Fac. of Sci. Helwan Univ. Cairo. Egypt.
Fawzy, R.N, Mahdy AMM, El-Mageed AMH. 1992. Identification of a strain of cucumber mosaic virus isolated from naturally infected Philodendron selloum. Annals of Agric. Sci. Moshtohor 30(3): 1219-1232.
Kiranmai, G, Sreenivasul P, Nayudu MV. 1997.Characterization of cucumber mosaic cucumovirus isolates naturally infecting three solanaceous vegatible crops in Andhra Prodesh. Indian Phytopathology 50(3): 416-425.
Lana, O, Agrios G.N. 1974. Properties of a strain of Tobacco mosaic virus isolated from white ash trees. Phytopathology 64:1490-1495.
Lecoq, H, Wisler G,Pitrat M. 1998. Cucurbit Viruses : The classics and The Emerging. INRA, station de Pathologie Vegetable, Domaine Saint Maurice, BP 94, 84143 Montfavet cedex. France.
Lee, S.Y, Park S.J, Choi J.K. 1997.Characterization of an ; isolate of cucumber mosaic virus from Forsythia (Forsythia koreana nakai). Korean Journal of Plant Pathology, 13(5): 358-363.
Lucas, G.B. 1975. Disease of Tobacco. 3rd Ed. Raleigh-North Carolinal Biological Consult Assoc.
Megahed, A.A. 2008.Effect of antiviral proteins produced by bacterial and fungal isolates on some viruses infecting vegetable crops [Thesis]. Faculty of Agriculture, Ain Shams University. Cairo, Egypt.
Mughal, S.M, Zidgali S, Matrooshi A.R. 2006. Some biological serological, and physical properties of Tobacco mosaic virus (TMV) from the sultanate of Oman. Pak. J. Agri. Sci. Vol. 43(1-2).
Nurhayati. 2012. Virus penyebab penyakit tanaman. Universitas Sriwijaya. Palembang.
Park, W.M, Ryu K.H, Choi J.K. 1990.Properties and purification of cucumber mosaic virus as new strain. Korean Journal of Plant Pathology 6(3): 393-401.
Shahwan, E.S. 2010. Inducing systemic resistance against some tomato virus diseases [Dissertation]. Moshtohor: Banha university.
Sugiura, M, Bandaranayake C.M, Hemachandra G.H. 1975. Chilli virus diseases in Sri Lanka. Technic Bull 8.
Taufik, M, Hidayat S.H, Sujiprihati S, Suastika G, Mandang S.M. 2007. Ketahanan beberapa varietas cabai terhadap Cucumber mosaic virus dan Chilli veinal mottle virus. Jurnal HPT Tropika 7 (2):130-139.
Wahyuni. 2005. Dasar-dasar virologi tumbuhan. Gadjah Mada University Press. Yogyakarta.
ZitikaiteI,J.S, Urbanaviciene L, Zizyte M. 2011. Cucumber Mosaic Virus Identification in Pumpkin Plants. Zemdirbyste=Agriculture 98 (4): 421‒426. ISSN 1392-3196.
DOI: http://dx.doi.org/10.33087/jagro.v8i2.215
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